Puc57 ampicillin sequence

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Puc57 ampicillin sequence
Plasmid: pUC57. Analyze: Sequence. Plasmid Type: Unspecified. Cloning
Method: Unknown. Size: 2710. Bacterial Resistance: Ampicillin. Notes: Cloning
vector …pUC57. Cloning vector with an ampicillin resistance marker, suitable for … To see
this sequence with restriction sites, features, and translations, please …pUC57 is a common used plasmid cloning vector in E. coli. The vector length is …
in lyophilized form. Forward Sequencing Primer, GTAAAACGACGGCCAGTG.Page 1. Page 2. Page 3. Page 4.PUC57-Simple Vector Map. Multiple Cloning Sites: M13F (-47). M13F. 5'C GCC
AGG GTT TTC CCA GTC ACG ACG TTG … PUC57-Simple Sequence (2710 bp):.Thermo Scientific Plasmid pUC57, 2710 bp in length, is a derivative of pUC19.
pUC57 MCS contains 6 restriction sites with protruding 3-ends that are resistant …Standard Vector Maps. pEX-A128 (Ampicillin), download, pEX-K168 (Kanamycin
), download. pEX-A258 (Ampicillin), download, pEX-K248 (Kanamycin) …Sequence map. "Cloning vector with an ampicillin resistance marker, suitable for
generating ExoIII deletions." The pUC57's MCS contain 6 restriction sites with …M13F(-47): 5'-d(CGC CAG GGTTTT CCC AGT CAC GAC)-3'. M13R: 5'-d(CAG
GAA ACA GCT ATG AC)-3'. Page 2. pUC57-Amp Sequence (2671 bp):.pUC57-Amp … It is high copy plasmid, with ampicillin resistance marker and
white/blue LacZ expression … 5 ug of purified plasmid vector; Sequence
information.pUC19 is one of a series of plasmid cloning vectors created by Joachim Messing
and … In addition to β-galactosidase, pUC19 also encodes for an ampicillin …
and host strains: Nucleotide sequences of the M13mp18 and pUC19 vectors".13 Mar 2017 … Finally, a Sanger consensus sequence approach restricted to the cat … The
pUC57 plasmid also carries the bla gene, conferring ampicillin …Plasmid label: LacZ. Prokaryotic resistance: ampicillin Amp. Cloned strain: DH5
alpha. Culture conditions: 37 centigrade, aerobic, LB. 5'sequencing primers: …How do I know that I am getting the correct sequence? AITbiotech has … What
restriction sites do you use when cloning synthetic genes into pUC57?bacterial genome, without introducing mutations in the coding sequence of the
resistance gene. …. pUC57 vector also contains a gene for ampicillin resistance.They showed a high degree of sequence homology to a known peptidase ……
promoter, this plasmid also contains the gene for ampicillin resistance (amp), a
MCS ….. addition of plasmid DNA was used, while pUC57 plasmids carrying a …For DNA sequence less than 1 kb, DNA sequence was broken into short
overlapping single-strand oligonucleotide … by gel purification and be cloned
into cloning vector (plasmid pUC57 as standard) by ligation or other methods. ….
Ampicillin.19 Aug 2017 … … coli with Optimized. Concentration of Ampicillin as Selection Marker … pUC57-
GFP was transformed into E. coli Top10F'. The expression of …pUC57 87. pYX-Asc 88. pZERO-2 89. pZL-1. Note: The sequencing primers listed
… Antibiotic: ampicillin … Sequencing Primers: M13(-21), M13 reverse, t7, sp6What is the function of this AAV2 ITR sequence in vectors? …. Sample 4 is the
plasmid I bought puc57 + cDNA after the restriction and BEFORE purification; …..
I got colonies on the LB agar plate (100ug/mL ampicillin), and I know that there is
 …

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